유전자공학 (6) 썸네일형 리스트형 Fertility loss in senescing Arabidopsis ovules is controlled by the maternal sporophyte via a NAC transcription factor triad 1. ovule degeneration proceeds in distinct successive senescence stages - ovule senescence stage (intact~stage3) - ovules in pistil (4DAE~6DAE) 2. the cells of the gametophyte degenerate sequentially during ovule senescence - pDD33>>HTA6-GFP : antipodals - pDD45::NLS-tdTomato : egg cells - pDD27::NLS-GFP : synergid and central cells - CC present > Regular SC nucleus 3. integument degeneration di.. LLG2/3 Are Co-receptors in BUPS/ANX-RALF signaling to Regulate Arabidopsis Pollen Tube Integrity 1. LLG and LLG GPI-APs are strongly expressed in pollen and pollen tubes 2. llg2 llg double mutants generated by CRISPR-Cas9 are male sterile * RNAi-mediated gene silencing 3. LLG2/3 coordinate pollen tube integrity In vitro and In vivo 4. LLG2/3 are involved in RALF4/19 - mediated signaling as co-receptors CRISPR/Cas9 - Based Gene Editing Using Egg Cell-Specific Promoters in Arabidopsis and Soybean 1. construction of an ECp-Cas9/gRNA system for genome editing in Arabidopsis and Soybean * Golden Gate cloning * Gateway cloning * T7E1 assay Central-cell-produced attractants control fertilization recovery 1. A synergid-independent attraction system allows fertilization recovery Figure1. Fertilization recovery is achieved by redundant pollen tube attractants - ccg, myb98 homozygous mutant - gcs1(hap2)mutant pollen ← CRISPER-Cas9 Figure2. Synergid-independent attractants salvage seed production - MYB98::H2B-TdTomato - immunostaining - pollen-tube-attraction ability of synergid-depleted ovules compe.. Mutations of the AtYAK1 Kinase Suppress TOR Deficiency in Arabidopsis 1. A genetic screen identifies mutations in AtYAK1 that suppress lst8-1-1 growth defects mutations in AtYAK1 - sol23, sol69 : suppressors the the lst8-1-1 mutant ← EMS - atyak1-3, atyak1-4, atyak1-5 : loss-of-function mutations ←T-DNA 2. Atyak1 mutations suppress the delay in flowering and reduced hypocotyl growth observed in lst8-1 mutants Atyak1 mutation : atyak1-4 A. flowered earlier B. incre.. 유전자 공학 개념 및 응용 1. 유전공학의 개념 유전공학 = "단백질 산물" 이용하는 기술 *유전공학에 필요한 기술* 유전자확보(cloning/isolation) 조작(manipulation) 발현 체계 구축(construction of expression systems) 형질전환체 생성(generation of genetic transformants) 2. 핵산의 성질과 분리 (1) central dogma 中 DNA duplication - RNA primer : 복제를 위해 중합효소가 붙어야 할 곳을 알려주는 짧은 염기서열 - DNA pol III : leading strand에서 5'→3'으로 복제할 때 쓰이는 중합효소 - DNA pol I : lagging strand에서 RNA primer를 제거하고 빈부분을 메꾸는 중.. 이전 1 다음
